Electroacupuncture preconditioning protects against myocardial ischemia reperfusion injury by modulating dynamic inflammatory response

BackgroundThe protective effects of electroacupuncture (EA) preconditioning against myocardial ischemia reperfusion injury (MIRI) have been reported. However, the underlying mechanism remains unclear. Recent research has indicated that dynamic inflammatory response following MIRI plays an essential role in progression injury. This study aimed to investigate EA on rats and explore relevant from perspective response.MethodsA model was employed, were subjected Neiguan for four days prior modeling. The effect evaluated by echocardiography, Evans blue triphenyltetrazolium chloride staining. Real-time polymerase chain reaction, Western blot, hematoxylin & eosin staining, immunohistochemistry utilized detect content mitochondrial DNA, NOD receptor family protein 3 (NLRP3) inflammasome activation, neutrophil recruitment macrophage infiltration blood samples myocardium below ligation.ResultsWe found could accelerate recovery left ventricle function after reduce infarction area, thereby protecting MIRI. Furthermore, observed ameliorate impairment, level plasma modulate NLRP3 attenuate infiltration, promote polarization M1 macrophages towards M2 MIRI.ConclusionEA mtDNA, suppress overactivation inflammasome, facilitate transition acute pro-inflammatory phase anti-inflammatory reparative MIRI, ultimately confer cardioprotective benefits.